Introduction: Neonatal sepsis is the one of the most important and a major cause of neonatal morbidity and mortality. Thrombocytopenia is a common manifestation in neonatal sepsis. Platelet indices change can be an early sign of sepsis and may correlate with its severity. Aims & Objectives: The aim of the study was to evaluate thrombocytopenia and variations in platelet indicesin neonatal sepsis. Materials and Methods:This cross-sectional observational research study was carried out at neonatal intensive care unit ofAdesh Medical College and Hospital, Shahbad.All neonates (<28 days) with clinically diagnosed neonatal sepsis were included except those who fell into exclusion criteria. Statistical tests (chi square, t-test) were applied using SPSS (Statistical Package for Social Sciences) version 21.0 software. Results:Out of 54 cases of neonatal sepsis with thrombocytopenia,Gam positive organisms associated with thrombocytopenia comprises of 40% cases whereas gram negative organisms associated with thrombocytopenia in 60% cases. 31 out of 54 cases (58%) with neonatal sepsis had increased values of MPV and 39 out of 54 cases (73%) with sepsis had increased values of PDW (73%).8 out of 39 cases with increased PDW values showed severe degree of thrombocytopenia. Conclusions: Thrombocytopenia,high PDW and high MPV are more associated with neonatal sepsis. So, platelet and its indices may be used as a sensitive marker to identify neonatal sepsis.