Aim: To compare the microscopic parameters for analyzing urine using automated and light microscopy and to establish the concordance between manual microscopy and automated urine sediment analyser, considering the increasing use of the later in medical laboratories compared to manual analysis.Material and Methods: The present study was done in the haematology section of Department of Pathology, MMIMSR, Mullana on all urine samples received in the laboratory during the period of 1 year from September 2022 to October 2023. It was an observational and correlation study done on randomly selected 509 urine samples.The study focused solely on microscopic parameters in urine analysis, including red blood cell (rbc), white blood cells (wbc), epithelial cells, casts, crystals and bacteria/fungus). All urine samples of 15 ml or more were included and those less than 15ml or containing preservatives were excluded from the study. Urine samples were taken into two tubes; one for automated analysis using the UF-4000 analyser and the other for manual microscopic examination, and the results were compared. Result: The average age of patients upon presentation was 44.18 years, with female predominance (57%) and male: female of 1:1.37. Majority of the patients (87.82%) came for a routine health check-up. On comparing the Sysmex 4000 and manual microscopy, we found that the Sysmex 4000 detected wbc, rbc and epithelial cells more accurately than manual microscopy. However, manual microscopy performs better in analysing the types of casts, crystals, and microorganisms (bacteria/fungus).Even though, the Sysmex 4000 provided the data on these microscopic parameters, but itcould not identify the type of cast or crystal, and categorize microorganism. Conclusion: We concluded that the red flag functionality was one of the advantages of Sysmex 4000 automated urine analyser. Although it gave specific counts for parameters such as RBCs, WBCs and epithelial cells, in contrast to manual microscopy reporting “full field” for large numbers. However, it only indicated the presence and number of casts, crystals, and bacteria but could not specify their type and morphology, where manual microscopy was required. The Sysmex 4000 took less time as compared to manual microscopy while analysing the urine samples.