Background: The present study was conducted for evaluating the prevalence of Urinary Tract Infection in Infants with Unexplained Acute Fever. Materials & methods: A total of 100 infants were enrolled. Complete demographic and clinical details of all the subjects was obtained. For calculation of point prevalence, all the 100 infants were divided into four study groups with 25 infants in each group as follows: infants aged less than 3 months, 3–6 months, 6–12 months, and children of 12 months to 24 months of age. Urinary tract infection was defined as detection of traces or higher levels of leucocyte esterase and/or nitrite in the test strips. Urine samples were obtained from all the specimens and were sent to laboratory for analysis. All the results were recorded and analysed using SPSS software. Results: Prevalence of urinary tract infection among subjects of age group of less than 3 months, 3–6 months, 6–12 months, and children of 12 months to 24 months of age was 48 percent, 40 percent, 32 percent and 20 percent respectively. Overall prevalence of urinary tract infection among infants with unexplained acute fever was 35 percent. Conclusion: Physicians need to carefully assess for UTI in infants with unexplained fever greater than 39 °C.