Background: Vitamin D deficiency can be seen with high incidence rate globally. Vitamin D deficiency complications are associated with various diseases. Anemia is one of the clinical conditions which may arise in vitamin D deficient individual. It was also found that Vitamin D has an effect on erythropoiesis. Aim of this study is to investigate the association if any, in between vitamin D and anemia. Methodology: Total 100 subjects were enrolled in this study after proper case taking. 50 subjects were healthy controls and 50 were enrolled as cases. Subjects were investigated for the levels of 25-hydroxy-vitamin-D and hemoglobin. Results: Anemia was present in 48.3% in cases having (25- hydroxyl-vitamin-D <30 ng/ml) and 25 % in controls having (25-hydroxyvitamin-D >30ng/ml) and the difference was statistically significant (p=0.01). Mean of Hb in cases was (9.62±1.06) and that was (10.66±1.50) in controls and the difference was statistical significant with (p=0.001). Conclusion: This can be concluded that patients with vitamin D deficiency are at greater risk of Anemia.