Background: Measurement of central corneal thickness (CCT) is highly useful for both diagnostic and therapeutic assessment purposes. CCT is helpful in diagnosis and progression of certain corneal disease such as Fuch’s corneal endothelial dystrophy.Present study was aimed to study Inter observer and intraobserver reproducibility of central corneal thickness measurements by using commercial available ultrasonic Pachymeter. Material and Methods: Present study was single-center, prospective, observational study, conducted in individuals of age 40 to 80 years, attending glaucoma clinic, irrespective of whether they have glaucoma or not. PACSCAN 300AP of Sonomed, an ultrasonic pachymeter is used for this study. All the subjects underwent pachymetry as described above by three observers. Results: In present study, among 40 subjects, 17 were female and 23 were male. All of them underwent pachymetry in both of their eyes, thus a total of 80 eyes were included in the study. The mean CCT of observer A, observer B & observer C was 532.8µm, 528.3µm & 530.1µm respectively. There is no significant difference between the three mean CCT measurements (p=0.7). By Bland–Altman plot, the limits of agreement are narrow with respect to the mean CCT, thus indicating excellent agreement. The mean measurement difference between observers was 2.91μm and the mean expected Inter observer measurement was within ±1.00% and the largest observed value was < ±1.1%. The ICC for reproducibility study was 0.979 (p<0.0001)suggesting that there is significant correlation between mean of the observer A, Observer B and Observer C. For the observer A, the ICC was 0.967(p-value<0.0001) suggesting there is a significant correlation between 3 CCT measurements. Conclusion: A variation of ≥15 mm between two repeated measurements occurred in 20.14% test-retest Inter observer evaluations, and in 30.93% test-retest Intra observer evaluations. This indicates that, despite the high reproducibility of the procedure.