Introduction: Enterococci are opportunistic pathogens that can cause various infections, especially in hospital settings. They are often resistant to many antibiotics, making them difficult to treat. Aim & objectives: This study aimed to evaluate the microbiological, epidemiological, and clinical profile of enterococcal infections at a tertiary care hospital in India. Methodology: We collected 11,846 clinical specimens from June 2022 to May 2023 and identified 100 enterococcal isolates using the VITEK-2 compact system. We also collected demographic and clinical data of the patients from the hospital information system. Result: The results showed that Enterococcusfaecium (64%) was the most common speciesfollowed byEnterococcusfaecalis (36%). Blood (67%) was the most common specimenfollowed by swab (13%). Diabetes mellitus (38%) was the most common comorbid conditionfollowed by chronic kidney disease (10%). The mortality rate was 26%. Tigecycline (100%) was the most sensitive antibiotic followed by linezolid (89%), vancomycin (86%) and teicoplanin (84%). Gentamicin High Level AminoglycosideResistance (HLAR) was 87% and vancomycin resistance was 14%. Conclusion: E. faecium was the most common speciesfollowed by E. faecalis.E. faecium was more prevalent in individuals aged >40 years. Gender did not significantly influence the distribution.E. faecium was dominant in blood specimens indicating its systemic nature. Clinical outcomes did not significantly differ between E. faecium and E. faecalis groups. Enterococcal infections were associated with high morbidity and mortality. E.faecium showed limited sensitivity to penicillin. E. faecalis showed higher sensitivity to nitrofurantoin in urine isolatessuggesting its potential efficacy in urinary tract infections.Antibiotics such as tigecycline, linezolid, vancomycinand teicoplanin were effective for both species and are recommended as empirical therapy.