Background: Osteoarthritis (OA) of the knee is a prevalent condition causing significant morbidity. Hyaluronic acid (HA) has been proposed as a therapeutic agent for its potential to improve joint lubrication and reduce symptoms. Objective: This study aims to evaluate the effect of HA injections on pain relief and functional improvement in patients with knee osteoarthritis. Methods: A prospective study involving 100 patients with knee OA was conducted. Participants received three weekly intra-articular injections of HA. Pain and function were assessed using the Visual Analog Scale (VAS) and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) at baseline, 1 month, and 3 months post-treatment. Results: Significant reductions in VAS scores and improvements in WOMAC scores were observed at 1 month and 3 months post-treatment compared to baseline (p < 0.05). Conclusion: HA injections provide significant pain relief and functional improvement in knee OA patients, suggesting it as a viable treatment option.