Introduction: Tuberculosis (TB) is a noteworthy medical issue far and wide, with a rough frequency of 9.4 million (8.9– 9.9 million) and with around 1.7 million TB-related passing deaths in 2009. determination of active PTB is over and over again late on the grounds that less than half of these patients have a positive sputum smear and isolation of Mycobacterium tuberculosis (MTB) takes quite a while. Aim of the study: Comparative study to detect the role of fiberoptic Bronchoscopy in sputum negative for pulmonary tuberculosis. Material and Methods:This study will be conducted as a prospective cohort study,Patient seeking medical attention at Sri aurobindo medical college and Post Graduate institute , Hospital ,during the period of study from august 2016 to may 2018. Descriptive statistics was used to show the characteristics of the collected sample. T-Test was used for quantitative measurement and for qualitative measurement Chi-square test applied. Results: Total 121 cases among 46 female and 75 maleswith no previous history of tuberculosis (fresh cases) ranging from 16 to 90 year of age and 24 to 73 kg of weight (mean of 46.19) were included, Maximum cases belonged to the age group of 46-55 yr of age (26) followed by 56-65 yr and more then 65 of age. Conclusion: This study enhance that FOB can give superb material to finding of associated cases with when smears of expectorated sputum don't uncover mycobacteria. Henceforth there is have to figure guidelines which are now undertaken in consideration where the respiratory doctor can make a complete conclusion without expanding the quantity of pointless treatment in new sputum negative tuberculosis patients.