Background: Subclinical hypothyroidism (SCH) is preliminary state of thyroid disorder, in which the circulating levels of Thyroid Stimulating Hormone (TSH) are slightly above the normal range (4.6-10 mIU/lit), whereas thyroxine (T4) and triiodothyronine (T3) are still within the normal limits. Iron deficiency is reported in patients of hypothyroidism. Thus, routine screening, and early detection of iron profile can be helpful to prevent the further progression of Subclinical to Overt hypothyroidism. The present study aims to detect the prevalence of iron deficiency in cases of Subclinical hypothyroidism.Methods: 50 patients of subclinical hypothyroidism (58% female and 42% male) and equal number of age and sex matched controls were included in the study. Thyroid Profile (TSH, fT3, fT4) and Iron Profile (Total Iron, TiBC, Ferritin) were analysed on auto analyser which is based on the chemiluminescence immunoassay. The collected data was analysed statistically.Result: Significantly low levels of serum iron was found in cases of subclinical hypothyroidism (p-value=0.0036). Serum iron levels (r= -0.448; p-value =0.0011) and Serum ferritin (r= -0.485; p-value =0.0003) showed a highly significant negative correlation with serum TSH levels.Conclusion: Based on the results of our study we conclude that there is a higher prevalence of Iron deficiency in SCH patients, hence we hypothesize that by routine laboratory testing, early detection of Iron profile and appropriate therapeutic measures can be helpful in preventing the onset of overt hypothyroidism.