Background: Strokes continue to rank as the second most common cause of death globally and the third most prevalent cause of disability. The present study was conducted to assess the association of ischaemic stroke and serum 25-hydroxyvitamin-D levels. Materials & Methods: 128 subjects were divided into 2 groups based on stroke. Group I had stroke and group II had not stroke. Parameters such as body mass index (BMI), marital status, education, and 25(OH)D levels (deficiency: <30 nmol/L, insufficiency: 30–50 nmol/L, normal: 50–125 nmol/L, and adequacy: >125 nmol/L) etc. were recorded. History of presence or absence of drinking, emphysema, chronic bronchitis, hypertension, high cholesterol, and diabetes mellitus was also recorded. Results: Group I had 44 males and 20 females and group II had 34 males and 30 females. There were 32 married, 18 unmarried and 14 divorced in group I and 38 married, 20 unmarried and 6 divorced in group II. Education was more than high school in 46 and 48 and less than high school in 18 and 16 patients in group I and II respectively. Chronic bronchitis was positive in 11 and 3 patients in group I and II respectively. Emphysema was positive in 14 and 5 patients in group I and II respectively. Hypertension was positive in 52 and 7 patients in group I and II respectively. Diabetes mellitus was positive in 48 and 6 patients in group I and II respectively. The difference was significant (P< 0.05). The mean 25(OH)D nmol/L level was deficient in 10 in group I and 7 in group II, Insufficiency in 10 in group I and 7 in group II, normal in 10 in group I and 7 in group II, and adequate in in 10 in group I and 7 in group II patients respectively. Conclusion: Serum 25(OH)D deficiency may be associated with an increased risk of stroke.