The thyroid dysfunction, which is quite prevalent in the population, affects many organs including male and female gonads. Hypothyroidism refers to a state that results in a deficiency of thyroid-hormones, including hypothalamic or pituitary disease and generalized tissue resistance to thyroid hormones. Thyroid-releasing hormone(TRH) can cause a rise in serum prolactin levels in the patients of hypothyroidism. The aim of the present study was to determine the correlation between the levels of Serum Prolactin and Thyroid Dysfunction.Materials and Methods: The present study was conducted on 100 newly lab. diagnosed and confirmed cases of hypothyroidism based on the lab. thyroid profile values and 50 healthy age and gender-matched controls. The levels of Serum T3, Serum T4, Serum TSH and Serum Prolactin were estimated in both the study group and in the control group.Results: The mean values of serum TSHlevels in the study group and in thecontrolgroup were 14.45±12.96µIU/ml and 1.27±1.05µIU/ml respectively (Normal value of TSH = 0.25-5.00 µIU/ml). The mean value of serum prolactin in the study group and in thecontrol group were 42.70±16.26ng/ml and 14.76±5.09ng/ml respectively (Normal value of Prolactin = 3.0-25 ng/ml). The levels of Serum Prolactin and TSH were increased in the study group as compared to the control group and statistically it was highly significant (p value <0.001). A positive correlation was found between the Serum TSH and Prolactin levels in the hypothyroid patients.Conclusion: In our study it was found that hypothyroidism leads to hyperprolactinemia, which further cause ovulatory dysfunction and consequently it leads to Infertility.