Aim:To analyze the biochemical profile of patients with jaundice at a tertiary care hospital. Materials and methods: 100 patients with serumbilirubin levels morethan2 mg/dl, Ageof18 years and above and Patients willing for laboratory investigation were included in this study. Detailed history along with demographicdetails was taken for all patients and a thorough clinical evaluation was performed. The participants’ bilirubin levels, aminotrans ferase, ALP, protein levels, PT/INR, haemoglobin levels, total counts, platelet counts, and creatinine level swere evaluated. Results: Outof100patients, 38patients(38%)werelessthan40years,44 patients(44%)wereaged60years andabove. Out of100 patients,78patients(78%)were males and 22patients(22%)were females. Out of 100 patients, 6 patients (6%) had transaminase level upto 30 u/l, 50 patients(50%)had ransaminase levels bet ween31-100u/l,38 patients(38%) had transaminase levels between 101-500 u/l and 6 patients (6%) had transaminase level between 501-1000 u/l. On the basis of the transaminase ratio, 83 patients (83%) had AST>ALT, 7patients(7%)had AST=ALT, 10patients(10%)hadALT>AST.61patients (61%) had normal ALP and 39(39%) had elevated ALP. Albumin was > 3.5 g in 50 patients (50%), 2.8to3.5g in 24patients(24%)and<2.8 gin26patients(26%).There is a statistically significant association between transaminase values and final outcome. Majority of patients with transaminase values above 500 had poor outcome. There is a statistically Significant association between ALP values and final outcome. The proportion of patients with poor outcomes were higher among the ALP elevated patients when compared to normal ALP patients. Conclusion: Timely and crucial assessment of biochemical markers is critical in patients with jaundice to avoid deterioration of their clinical condition and death.