Objective: Self-medication(SM) is the self-administration of a treatment without a prescription from a physician. Present study was conducted to know the prevalence of self-medication among medicalstudents at Gwalior. Methods: A cross-sectional study was conducted with the participation of undergraduate medical students by using a short, self-administered questionnaire. Data was collected and analyzed for counts and percentage. Chi Square test applied and Odds ratio with their CI were calculated. P value was judged at 5% level of significance. Results: Total 285medical undergraduate students were participated in this study; the prevalence of self-medication was 69.8 %. The prevalence of medicine use was significantly same among males and females. Majority of the participants were taking SM as they think illness was mild in nature (72.4%). About half (53.3% and 52.8% respectively) were taken SM from the Old prescription for same illness and consulting their seniors and friends. The most frequent symptoms and illness reported by students who practiced self-medication were headaches (94.5%) followed by coughs (40.2%). Conclusion: The use of medicines, self-medication was found high among the undergraduate medical students. This underscores a need for advocacy on responsible self-medication practice during the formal training of these future health professionals, in order to avert its widespread negative consequences.