Background and Objective: Deficiency of Vitamin D is very common and same applies to Hypothyroidism in Indian population, Impact of Vitamin D deficiency on Thyroid functions and data on its effect on patients with hypothyroidism is limited. The present study was conductedin hypothyroid patients to know the relationship between serum Vitamin D and calcium levels with Thyroid hormones. Materials and Methodology: Tertiary Care Hospital based study was conducted to estimate and corelate the levels of Serum Vitamin D, Calcium and Thyroid hormones in 30 cases of hypothyroid and controls. Analysis was done using SPSS version 14.0 statistical software. Results: There was statistically significant correlation seen between low Vitamin D levels and Low TT3 levels.Serum Calcium levels were low in patients with hypothyroidism. Conclusion: Estimation of serum Vitamin D levels and serum calcium levels in Hypothyroid patients may be of significant help as it may suggest requirement of prophylactic supplementation with Vitamin D to reduce the cardiovascular and other risk causing increased morbidity in these patients.