Introduction: Preterm birth is a leading cause of neonatal morbidity & mortality worldwide & is estimated to complicate approximately 10-12% of pregnancies. The concept of anterior Uterocervical Angle can add more possibility to diagnosing preterm birth. Materials and Methods: It is a Prospective Observational study conducted in the Department of Obstetrics and Gynaecology, Kamla Raja Hospital, G.R.M.C, Gwalior, M.P during a period of January 2021 to June 2022. The aim of this study is to measure uterocervical angle by transvaginal sonography between 12-24 week of gestation for prediction of spontaneous preterm birth, Resuts: Recently, the potential of UCA as an ultrasound marker to predict sPTB has been identified. In this study, UCA in the second trimester was significantly wider in the preterm group compared with the term group and there was a significant association between obtuse UCA and risk of spontaneous preterm labor. Discussion: In the present study 69.7% women had anterior uterocervical angle <95 degree while 30.3% women had angle more than >95 degree. Out of 85 women measuring angle <95 degree 90.6% women had term birth and 9% women had suffered preterm birth. Obtuse anterior uterocervical measurement >95 degree was observed in 37 women out of which 29 women (78%) had spontaneous preterm birth (<37week). The obtuse uterocervical angle further divided in two groups having angle between 95- 105 and > 105. Out of 29 people 22 women (75%)were delivered preterm having angle between 95-105 while out of 8 women with angle >105, 7 women (90%)had preterm delivery. Conclusion: This study suggests that the UCA is a potential novel screening tool for the prediction of sPTB. However, detection rates of sPTB can further be improved if combined with other parameters such as CL, maternal demographic and obstetric history.