Background:Vulvovaginal candidiasis (VVC) is a common fungal infection affecting women globally, with increasing recognition of non-albicans Candida species as significant contributors, particularly among diabetic individuals. This study aimed to investigate the clinical and mycological aspects of VVC, focusing on Candida non-albicans prevalence and its association with diabetes mellitus.Methods:A prospective study was conducted at the Institute of Venereology, Madras Medical College & Rajiv Gandhi Government General Hospital, Chennai, from April 2021 to March 2022. Female patients aged 18-60 years with VVC were included, and clinical and mycological assessments were performed.Result:Of 100 participants, 55% had diabetes mellitus. Candida glabrata was significantly more prevalent among diabetic patients (34%) compared to non-diabetic patients (4%) (P < 0.001). Itching over genitalia was significantly more common among diabetic patients (73%) compared to non-diabetic patients (44%) (P = 0.004).Conclusion:The study highlights the importance of considering non-albicans Candida species, particularly Candida glabrata, in the diagnosis and management of VVC, especially among diabetic individuals. Comprehensive clinical assessment and management strategies are crucial in this population.