Aim and Objective: To find out Role of MMP9 and TIMP2 gene polymorphisms in the varicose veins formation in North Indian population. Methods: A total of 600 (300 cases and 300 controls) individuals were selected for the study, who had diagnosed for varicose veins complication from January 2020 to July 2020 at Government Medical College, Jalaun (Orai) UP. (India). Cases and controls age was between 10-60 years. Fresh 5 ml of EDTA blood was drawn for genetic analyses from both patients and controls as per standardized protocols. For the identification of MMP-9 and TIMP-2 genes polymorphism, DNA were isolated from the fresh blood by using Qaigen kit protocol. PCR-RFLP was performed to determine genotype variants. Results: The 50% males and 50% females were selected for this study. 59 (19.66%) cases were reported those had family history of varicose veins formation while it was found quite less 8 (0.02%) in the controls with OR=44.22, 95% Cl=14.78, p-value <0.0001. In the PCR-RFLP the distribution of genotypes frequencies in the cases and controls groups it has found that gene frequencies of MMP-9 genotypes have not found statistical significance, whereas the gene frequency of TIMP-2, GG (homozygous) genotype was found statistically significant which was higher in the controls than in the cases. Conclusion: To our knowledge, this is the first study to report on MMP-9 and TIMP-2 gene polymorphisms in relation to varicose veins formation in north Indian population. In our sense, such type of studies are required to know the strength and exact nature of the genetic variation to know which of the variant within a haplotype cluster could be functionally related to varicose veins formation in the populations. This study may have demonstrated a statistically significant result in the studied population in large group.