Background: Blood is a vital component of the human body, playing a crucial role in maintaining overall health and well-being. The importance of TTI testing cannot be underestimated as HIV, HBV,HCV, and SYPHILIS are transmitted by blood. Aim: To study the seroprevalence of HIV, HBsAg, HCV, and VDRL in blood donors. To compare the seroprevalence of these diseases in male and female donors. To compare gender and age-wise seroprevalence. Methods:The study was conducted in the blood bank, Department of Pathology, GMERS Medical College,Morbi, India from JANUARY 2022 to DECEMBER 2023. Blood donors who came to the blood bank or participated in a camp for voluntary blood donation provided samples of their blood. We collected data from total a total of 2734 donors. Results:A total of 2734 donor samples were analyzed during the said period.Out of 2713 accepted donors, a total of 21 (0.76%) were diagnosed positive for TTI infection. Conclusion:It is mandatory to reduce the incidence of transfusion-transmitted diseases in India by implementing rigorous donor selection procedures, including comprehensive screening for HIV, HBV, HCV, and VDRL as well as by using sensitive laboratory screening tests.