Background: Gender preference and the practice of female foeticide continue to be significant social issues in many parts of the world. Understanding the knowledge and attitudes towards these issues among the unmarried population of reproductive age is crucial for developing effective interventions. Objective: This study aims to assess the level of knowledge and attitudes regarding gender preference and female foeticide among the unmarried population of reproductive age. Material and Methods: A cross-sectional study was conducted among unmarried individuals aged 18-45 years. Data were collected using a structured questionnaire, which included questions about demographic information, knowledge about gender preference, attitudes towards female foeticide, and sources of information influencing these attitudes. Descriptive statistics and inferential analysis were used to examine the data. Results:A high level of awareness about what female foeticide is, was observed among both female (93.18% high awareness) and male (96.29% high awareness) participants. Knowledge on how sex determination is conducted showed some variation, with a higher percentage of females (73.86% high awareness) compared to males (72.84% high awareness) having high awareness, and more males (24.69%) than females (14.77%) having medium awareness. Awareness of places where sex determination is conducted was very high among both genders, with 95.45% of females and 98.77% of males having high awareness. The most cited reason for female foeticide and gender preference is poverty, with 40.8% of participants identifying it as a primary factor. Conclusion: The findings of this study shed light on the need for targeted educational and awareness programs to address gender biases and misconceptions about female foeticide among the unmarried population of reproductive age. The study underscores the importance of involving this demographic in efforts to combat gender discrimination and promote gender equality.