Aim: To evaluate correlation between peripapillary retinal nerve fiber layer thickness (RNFL) and optic nerve head rim area (RA) measured using spectral domain optical coherence tomography (OCT) with visual field indices using Humphrey visual field analyzer in Glaucoma patients and normal subjects. Methods: A prospective and observational study was conducted on 60 eyes of patients diagnosed with primary open angle glaucoma between the age group of 40-65 years and 60 eyes of age and gender matched healthy participants underwent ONH rim area analysis and peripapillary RNFL thickness measurements using spectral domain OCT and visual field indices determined using Humphrey perimetry. The study was conducted at the department of Glaucoma of a tertiary eye care hospital for a period of one year. The correlation of the average RNFL thickness and rim area with the Visual field indices was determined using the Spearman’s correlation coefficient analysis and scatter plot. Results: A total of 60 glaucomatous eyes and age –gender matched 60 healthy subjects were included in the study. There were 36 males and 24 females, with ages ranging from 45 to 60 years. In Glaucoma patients, average RNFL thickness, RA, mean deviation MD in decibels and VFI were 60.45 +/- 10.87 μm, 0.70+/-0.27 mm2, -7.11+/-7.17 dB and 83.02 +/- 20.1 %, respectively. In normal subjects, average RNFL thickness, RA, mean deviation MD in decibels and VFI were 88.80+/-7.31 μm, 3.54+/-18.17 mm2,0.05+/-1.53 dB, and 99.27+/-1.23%, respectively. there was significant difference in OCT and VF parameters between normal and glaucoma cases (P<0.001).In Glaucoma patients, the average RNFL thickness (r = 0.236) showed a stronger positive correlation with VFI than RA (r = 0.143), but the difference was not statistically significant. Conclusion: In our study, the average RNFL thickness and rim area of the spectral-domain OCT demonstrated a positive correlation with the VFI of the Humphrey Visual Field Analyzer.Both structural and functional aspects should be evaluated in order to obtain full characterization of glaucomatous damage for clinical judgement and treatment.