Background: Acute febrile dengue fever is an arbo-viral illness that mostly affects tropical and subtropical regions worldwide. Over the past 50 years, the disease's incidence has grown, and 2.5 billion people now live in dengue-endemic areas. This study aims to assess the NS1 Ag test as a novel diagnostic tool for dengue virus infection early detection in a tertiary care setting. Materials and Procedures: From January 2023 to June 2023, this study was carried out in a tertiary care centre at Index Medical College Indore. Serum samples from 500 patients collected. Result: 500 suspected dengue fever samples were collected, of which 80 patients were found to be NS1 antigen positive. Conclusion: An intensive laboratory-based disease surveillance strategy that can offer early warning of approaching epidemic transmission is necessary for the effective prevention and control of the dengue outbreak.