Total 147 isolates were recovered from saline soil of Vidarbha region. Out of which 87 isolates (59.18 %) showed an antibacterial activity against test bacteria by agar overlay technique. In secondary screening, out of 87 primary isolates 19 actinomycete isolates were subjected for an antibacterial activity against test bacteria by agar well diffusion method. Nearly 78.94% isolates recorded antibacterial activity study towards S. aureus followed by 68.42% isolates to Bacillus subtilis, 63.15% for Streptococcus pyogenes, 52.63% to Escherichia coli and Proteus vulgaris, 47.36% towards Klebsiella pneumoniae, 42.10% for Pseudomonas aeruginosa and Salmonella typhi and 36.84% for Enterobacter aerogenes. Promising actinomycetes isolate (strain N8) showed antibacterial activity against all the test bacteria and was selected for morphological, cultural, physiological biochemical and genetic characterization. The 16S rRNA amplification for phylogenetic study revealed that the isolate (N8) was highly related to Streptomyces geysiriensis (100 %).