Objectives: The purpose of this study was to evaluate and compare the ability of Hank’s balanced salt solution (HBSS), Low fat milk, Soymilk, and Aloevera extract to maintain human periodontal ligament (PDL) cell viability in vitro. Method: PDL cells were obtained from extracted healthy premolars and cultured in Dulbecco’s modified Eagle’s medium (DMEM). The cultures were exposed for 1, 3, 6, 9 and 24 hours to experimental solutions (DMEM was positive control). Cells in the different media were examined under the optical microscope and their viabilities were analyzed by MTT assay. Results: The data were statistically analysed using Kruskal Wallis ANOVA and Mann Whitney ‘U’ test. Statistical analysis showed that the efficacy of Soymilk on PDL cell viability at 3hours was significantly higher than HBSS, low fat milk, Aloevera extract and control Group (DMEM) (p<0.05). Performance of low fat milk and Soy milk was equal to control group (DMEM) at 6, 9 and 24 hours. HBSS and Aloevera maintained significantly less viable PDL cells compared to Low fat milk and Soy milk. Conclusion: The results indicate that Soy milk and Low fat milk can be used as a suitable medium for avulsed tooth up to 24 hours.