Background: An ear infection is among the leading causes of deafness in many low/middle-income countries. Pseudomonas aeruginosa (P.aeruginosa),Klebsiella pneumoniae (K.pneumoniae), Proteus species, Haemophilus influenzae (H.influenzae) and Moraxella are the most common aerobic microbial species isolated from patients with otitis media and otitis externa. The present retrospective study was conducted for evaluating the bacterial etiology of ear infection and its antimicrobial susceptibility pattern in children. Materials &Methods: A total of 100 children with ear infections of bacterial etiology were enrolled. Complete demographic and clinical details of all the subjects were obtained. Subjects with ear infection other than that of bacteriological etiology were excluded. Data collection was done by means of a structured questionnaire which was administered to the participants. Before being processed at the central pathology laboratory, the collected specimens were stored in Stuart's transport medium at room temperature. A combination of selective and non-selective media, including chocolate agar (CA), sheep-blood agar, MacConkey agar (MCA), and Sabouraud dextrose agar (SDA), were used to inoculate each specimen. Isolates of bacteria were detected. This was followed by antimicrobial susceptibility testing (AST) using the Kirby-Bauer disc diffusion method.Results: Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella spp, Acinetobacter spp and Enterobacter spp. were found in 35 percent, 32 percent, 22 percent, 8 percent and 3 percent of the patients respectively. Staphylococcus aureus was susceptible to gentamicin, ciprofloxacin, clindamycin and erythromycin. Pseudomonas was susceptible to amikacin, gentamicin and ciprofloxacin. Klebsiella spp was susceptible to amikacin, gentamycin and ciprofloxacin. Conclusion: The current research indicates that ear infections are caused by several microorganisms that are resistant to drugs. Antimicrobial susceptibility testing is therefore essential for directing physicians in the proper management of ear infections.