Aim:The aim of this study was to investigate the impact of antibiotic prophylaxis on preventing postoperative infections subsequent to impacted mandibular third molar extractions. Materials and Methods: The study included patients aged 18 to 40 with no comorbidities and at least one impacted mandibular third molar. 120 patients were randomized into two study groups; Study group (prophylactic antibiotic group) and control group (Placebo group). Results: A total of 120 patients were enrolled and randomly assigned to two groups. Baseline characteristics of participants were assessed, and no significant differences were observed between the two groups. Incidence of postoperative complications was 4 patients and 6 patients among study group and control group respectively. On comparing, non-significant results were obtained. Conclusion: The administration of 250mg amoxicillin one hour prior to surgery did not demonstrate significant effectiveness in reducing the risk of postoperative infections following impacted mandibular third molar extraction.