Introduction& Aim: Early childhood caries, often known as nursing bottle caries, increases the risk of caries throughout the permanent dentition and lowers quality of life children. This study was contemplated to assess the knowledge, attitude and practices about nursing bottle caries among mothers of pre-school children. Methodology: This cross sectional study was conducted among the 300 mothers of Pre-school childrenwho visited the Out Patient of Pedodontics department of Ragas Dental College and Hospital were recruited for the study conducted for a period of May 2023 to November 2023. Pre validated Questionnaire consists of three parts(Knowledge, Attitude and Practices), each part consist of 6 closed end questions. This investigator administered questionnaire was distributed the participants and responses were collected and analyzed by using SPSS software. Results: Mean age of the participants was 25.44 ± 3.48. Among the total mothers. 52.6% were from rural background64.9% of the mothers were aware the term nursing bottle caries, 65.5% of the mothers doesn’t know when to consult the dentist for very first consultation, 46.7% of mothers bottle fed their children more than two years. 92% of the mothers used bottle as a pacifier when your baby falls asleep. Discussion and Conclusion: Based on our study results, it was concluded that 64.3% mothers of preschool children’s were aware about nursing bottle caries. But the attitude towards nursing bottle caries and various practices methods to prevent the nursing bottle caries was significantly less.