Structures or functions that are aberrant at birth are referred to as congenital anomalies or malformations. 7% of infant fatalities are caused by congenital abnormalities. During pregnancy, trace elements may have a significant impact. Although copper deficiency and occasional excess are linked to a number of illnesses, it is unclear how these factors affect foetal development in utero. Quantitative analysis of trace element copper can identify whether cu deficiency or cu excess is related to congenital abnormalities. This study aimed to compare the copper (Cu) content in serum samples from mothers carrying healthy and congenitally deformed foetus. In the current study, 200 antenatal women participated, with 100 of them carrying a congenitally deformed foetus and 100 of them carrying a normal foetus at a comparable gestational age. A history and clinical examination were performed on all antenatal women. Blood samples were taken, and copper levels in µg/dl were determined using a colorimetric kit. Based on Quantitative analysis, the difference between the mean serum copper levels in the both groups was statistically significant (p 0.001), and it was 110.3+23.9 g/dl and 208.6+29.6 µg/dl, respectively with a normal reference range is 80-155µg/dl. The odds ratio found to be more than 1. The development of foetal congenital abnormalities may be significantly influenced by copper deficiency during conception or embryogenesis. The quantitative serum concentration of copper is of diagnostic significance in depicting congenital aberrations.