Background: Hepatitis C virus (HCV) infection is considered one of the growing public health problems. Identification of HCV associated risk factors is pivotal for developing appropriate prevention strategies. Aims & objective: This study determine the prevalence of HCV infection and their associated risk factors in our. Material & method: This cross sectional study was conducted in the Department of Microbiology, M G M Medical College, M.P. A total of 500 Blood samples were received during the study period in serology section for detecting Hepatitis C virus infection. The patient socio-demographic data (name, age, sex and address), Inpatient/outpatient number, risk factors, history of blood transfusion and clinical history were noted. Results: The prevalence of HCV infection was 4.4% (22/500). Majority of the patients 26.8% in total and 50% in HCV positive cases were 21-30 years age group. Among total cases 69.4% were males whereas among HCV positive patients 77.27% were male. The risk factor for HCV transmission was observed as blood transfusion (81.8 %), haemodialysis (4.54%), unsafe injection (9.09%) and IV drug abuse in only one case (4.54%). Conclusion: The study identified multiple risk factors for acquiring HCV infection and suggests appropriate interventions targeting high-risk populations