Background and Objective: Salmonella enterica serovars Typhi (S. Typhi) and Paratyphi (S. Paratyphi) A, B, and C are the pathogens that cause enteric fever, also known as typhoid and paratyphoid fever. Most cases of enteric fever are spread by the fecal–oral route. This study is to evaluate the isolation and identification of Salmonella spp. in suspected cases of typhoid fever. Material method: The current study was conducted in the Mahatma Gandhi Memorial Medical College's microbiology department in Indore, Madhya Pradesh, central India, between 2020 and 2021. Blood samples from suspected enteric fever cases were received by the microbiology department. Result: Out of the 140 blood culture samples that were obtained, 26 strains of Salmonella enterica that were positive for culture were identified and isolated. All 26 isolates of the Salmonella enterica species were found to be serovar Typhi. We did not isolate S. Paratyphi in this investigation. Conclusion: It has been determined that younger age groups and men are more susceptible to enteric fever. This study indicates that a common cause of Salmonella infection is Salmonella enterica serovar typhi.