Background and Objective A rising percentage of patients with impaired immune systems are being diagnosed with fungal respiratory infections more frequently. The majority of the time, colonisation is the initial stage of a respiratory fungal infection. The objective of this study is to isolate and identify filamentous fungi from different clinically suspected respiratory samples. Material method This study was designed to identify filamentous fungi species from all respiratory samples. Sampling was conducted from 2021 to 2023. A total of 28 clinical isolates of molds species were obtained from the respiratory samples of both immunocompromised and immunocompetent patients. The samples were first observed under a KOH mount and inoculated on SDA. The growth was identified using standard microbiologic methods.Result In this study, out of all respiratory samples, 4.52% were culture-positive for mold isolates. Aspergillus species (21 isolates) was the most common isolate, followed by Pennicillum spp. (2 isolates), Rhizopus (2 isolates), Fusarium (1 isolate), Trichothecium spp. (1 isolate), and Acremonium spp. (1 isolate). Conclusion We have concluded that the Aspergillus species is commonly involved in invasive respiratory infections, has emerged worldwide, and represents a serious public health concern. Early diagnosis is crucial for the successful management of patients.