Background:An anterior cruciate ligament (ACL) injury is a common knee injury, especially among athletes who participate in sports that involve sudden stops, changes in direction, or jumping. The present study was conducted to assess autologous peroneus longus and hamstring tendon for anterior cruciate ligament reconstruction in knee. Materials & Methods:80 patients with ACL injury of both genderswere divided into 2 groups of 40 each. Group I undergoing autologous peroneus longus tendon and group II undergoing hamstrings tendon graft in ACL reconstruction.Both the group of patients were regularly followed up in post-operatively 1 month, 3 months and 6 months and outcomes were measured via IKDC score Results: Group I had 22 males and 18 females and group II had 24 males and 16 females. The right side was involved in 23 in group I and 19 in group II and the left side in 17 in group I and 21 in group II. IKDC score at 1 month was 57.1 in group I, and 58.6 in group II, at 3 months was 66.9 in group I and 67.4 in group II, and at 6 months was 91.2 in group I, and 90.5 in group II. The difference was significant (P< 0.05). Conclusion: The donor site morbidity and post-operative knee functional score were similar for both groups.