Background: Asymptomatic bacteriuria (ASB) is defined as a pure culture of at least 105 organisms/ml of urine in the absence of symptoms. It is the most common bacterial infection requiring medical treatment in pregnancy. The present study was conducted for evaluating microbiological Profile of Asymptomatic Bacteriuria in Pregnancy. Materials & Methods: A total of 500 pregnant subjects were screened in the present study. Socio-demographic data were obtained. Clean-catch midstream urine was collected from each patient into a sterile universal container. Samples were cultured on dried plates of blood agar and cysteine lactose electrolyte deficient agar. Plates were incubated aerobically of 37°C overnight. Colony counts yielding bacterial growth of 105/ml or more of pure isolates were regarded as significant for infection. The isolated organisms from culture plates were identified by standard laboratory techniques. All the results were recorded in Microsoft excel sheet and were subjected to statistical analysis using SPSS software. Results: A total of 300 subjects were analyzed. ABU was present in 33.33 percent of the subjects. The mean age of the subjects with ABU was 29.3 years. The majority of the subjects were of rural residence. Escherichia coli, Staphylococcus aureus, Klebsiella species, Proteus species and Pseudomonas aeruginosa species were seen in 33 percent, 28 percent, 15 percent, 12 percent and 12 percent of the patients respectively. Conclusion: Significant proportion of pregnant subjects are affected with ABU. This is worrisome because UTI in pregnancy may have serious consequences for both the mother and the child.