Iron deficiency in infancy is widely related to several clinical and developmental problems such as delayed auditory brainstem response maturation, memory and behavioral abnormalities and several other neurodevelopmental deficits. This was a hospital based cross sectional study conducted with prior clearance from the Institutional Ethics committee (IEC) of this tertiary care hospital. All children who met the inclusion criteria during the study period after due consent from parents underwent the following: Hospital records were studies to for further information regarding morbidity, treatment details, nutrition during hospital stay, amount of blood drawn for investigations during NICU etc. Among the population evaluated mean of Hb was 11.03 gm/dL (max 13.4 and min 8.3) and serum ferritin was 43.94 μg/L (max 181.3 and min 10.3). As per postnatal age and weight defined criteria 20% (26 out of 131) developed anaemia while on prophylactic iron supplementation. However, 65% of the iron supplemented study population (85 out of 131) still had adequate ferritin levels (20 mcg/dL) at 3 months corrected age.