One of the important factor in the host response to tumor is the lymphocytic infiltration, along the tumor front which provide an immunologic barrier to spread of tumor. This barrier can be affected by the release of cytokine factor released by the tumor which lead to increased cortisol levels in the serum. Patient’s clinical parameters were recorded which include age, sex, tumor location, tobacco and alcohol use and medical history. Through clinical examination in which the site of the lesion and the extent of the lesion were assessed. The clinical staging was assessed by AJCC TNM staging.Tumor size was divided into T1, T2, T3 & T4 stage and mean serum cortisol level is calculated in each group. There were no patients in T1 stage. In T2 stage mean serum cortisol level was 9.15 with SD +_ 1.94. In T3 stage mean cortisol level was 15.58 with SD +_ 2.75 and in T4 stage mean serum cortisol was 20.65 with SD ±1.07. Cortisol levels was compared with clinical tumor size using kruskalwallis ANOVA test. Significant change in cortisol levels were found with the tumor size since p=0.0001i.e.p<0.05. Pair wise comparison by Man-Whitney ‘U’ test was done within the group which showed a significant change in cortisol levels with tumor size. Since p=0.0062 i.e., p<0.05.