Background& Objectives:Urinary tract infection is one of the most common infectious disease found in adult population and has become a major health problem. The urinary tract infection also affects commonly paediatric population especially in the age group of 0-14 years.Aim and objective: To find out prevalence of UTI in different age groups, and gender and to identify causative agent of UTI in children below 14 years of age.Methods:The present study was conducted in Paediatric department of Nalanda medical college and hospital Patna from August 2019 to July 2021. This cross sectional study noted epidemiological parameters like different age groups, sex, and bacterial isolates in paediatric population below 14 years of age.Result:Among total 1248 urine samples processed, 618 (49.52%) samples were collected from male children while 630 (50.48%) samples from female children. Of 1248 samples, only 306 (24.51%) samples were culture positive and hence UTI. Of 306 UTI cases, UTIs were higher in female children, i.e. 191 (62.42%) compared to male children, i.e. 115 (37.58%). UTIs were highest in age group 2–5 years 45.42%. Rate of UTIs in inpatients and outpatients were similar with slightly higher rate in IPD patients. Total of six different species/sets of bacteria were isolated. Among them, E.coli, 170(55.56%) was predominant followed by Klebsiella spp 48(15.69%).Conclusion:The most common age group affected by UTIs in paediatric population was 1-5 years. UTIs affect females more than males due to variable reasons. In present study E. coli followed by Klebsiella was most common bacterial isolates affecting UTIs in children.