Background & Aim: Patients admitted to the Intensive Care Unit (ICU) are prone to develop nosocomial infections due to Multidrug-Resistant (MDR) organisms. Inappropriate and overuse of antibiotics play an important role in the emergence of MDR organisms, which cause life-threatening infections resulting in significant morbidity and mortality. Our aim is to study the incidence of nosocomial infection, site of infection, pathogens involved and their susceptibility. Methods: Patients those who were admitted to intensive care unit for more than 48 hours were included. Clinical samples like blood, urine, sputum, wound/pus swab, intravenous catheter tips, endotracheal aspirates, urinary catheter, central venous catheter, inter costal drainage catheter tip were collected and sent for culture and sensitivity. Result: Out of 350 patients, 70 patients had nosocomial infection. The overall nosocomial infection rate was 20%. Most common infections were bloodstream infections followed by pneumonia and urinary tract infections. Nosocomial infection related mortality was most commonly due to pneumonia. Staphylococcus aureus were the most common blood stream isolates. Pseudomonas aeruginosa and Acinetobacter baumanii were the most common species found in pneumonia. Conclusion: The presence of nosocomial infection was associated with prolonged period of hospitalization and use of invasive devices which is associated with increased mortality and morbidity and increased cost of health care.