Background: The Government of India implemented medical waste management rules in 1998 to address the environmental burden and potential risks associated with hazardous biomedical waste. This study aims to assess the knowledge, attitude, and practices of biomedical waste management among medical, nursing students, and healthcare staff in a tertiary care hospital. Methodology- The study included 430 participants, including medical students, nursing students, sanitary workers, nursing staff, and doctors. The majority of healthcare personnel were trained in biomedical waste management, but training was lacking for sanitary staff and lab technicians. Only 18% of the participants were aware of the origins of the Biomedical Waste Act. Nurses and doctors had the highest levels of knowledge, followed by nursing and medical students. Result- Among all the healthcare workers, doctors (100%) and medical students (90%) had better awareness regarding the effects of improper disposal of BMW. Conclusion- Biomedical waste management practice was not up to the mark among healthcare workers which increases the risk of transmission of diseases. Therefore, it was determined that it is better to provide in-service training programs on biomedical waste management and upgrade the educational level for healthcare professionals.