Background: To study the folic acid supplementation on level of homocysteine in children on antiepileptic medication. Materials & Methods: A total of 40 participants were included in the study, with 30 assigned to the experimental group and 10 to the control group. Pearson correlation test was employed for assessing correlations, and a significance level of P < 0.05 was considered.Results: Forty participants were included in the study, and there were no significant differences in baseline data between the two groups. Throughout the follow-up period, no serious adverse reactions were observed.Conclusion: The administration of folic acid supplementation in children undergoing antiepileptic drug (AED) treatment may lead to a decrease in total homocysteine (tHcy) levels, potentially mitigating the risk of cardiovascular disease (CVD).