Background: The role of free Iron in Diabetes Mellitus is increasingly being recognized. It inhibits glucose production by the liver and decreases insulin metabolism causing peripheral hyperinsulinemia1. Iron released from ferritin undergoes a Fenton reaction to generate reactive oxygen species which damage the biological macromolecules.2 Material and Methods: A cross-sectional study to compare the levels of fasting plasma glucose, sr. ferritin, HbA1C, and lipid profile in newly diagnosed 100 type 2 diabetes mellitus patients with 51 age and sex-matched healthy controls was carried out from November 2013 to June 2015 at MR Medical College, Kalaburagi after excluding patients with type 1 diabetes, Hemochromatosis, Thalassemia, and patients on Iron supplementation. The unpaired 't' test was used to compare the biochemical parameters between cases and control. ROC curves were plotted for sr. ferritin to find the diagnostic accuracy and area under the curve. Data was analyzed using SPSS 22 and P<0.05 was considered statistically significant. Results: Sr. ferritin in males was 457.9± 402.2 ng/ml and 184.46 ± 36.47ng/ml in females while in controls it was 84.6±36.8 ng/ml (P<0.001). The mean FBS was 181 in males and controls was 98 mg/dl. There was no significant association between Sr. Ferritin and Diabetic Nephropathy, Retinopathy and Neuropathy. Conclusion:The present studyshowed a significant increase in sr. Ferritin,Suggests oxidative stress as one of the major factors for pathogenesis in Type 2 diabetes mellitus patients.