Background: Transfusion transmitted infections (TTIs) remain a major threat to the patients receiving blood. Accurate estimates of risk of TTIs are essential for monitoring the safety of blood supply and evaluating the efficacy of currently employed screening procedures. Objectives: Aim of this study is to estimate the incidence of transfusion transmissible infections (TTIs) among blood donors and analyse the effectiveness of screening methods for its prevention and control strategies. Methods: This was a cross-sectional observational study, enrolled 600 blood donors who donated blood during the study period. All the received blood units were screened and analyzed for TTIs. Socio-demographic data were also collected from all the participants. Screening of HIV, HBV, HCV, Syphilis and malaria in all blood units was done by appropriate methods. Results: Out of total donors, 250 (41.6%) were 18-30 years of age group and predominantly were males (65%).The voluntary blood donors were 55% and those who donated blood for the first time were 63.3%. Highest number 277 (46.2%) belonged to blood group “O positive”, followed by 138 (23%) with “B positive” blood group. The overall sero prevalence of TTIs was 2.83%. In sero-reactivity donors, maximum 8 (1.3%) cases were reactive for HBsAg, 5 cases (0.83%) for anti-HCV, 2 cases (0.33%) for syphilis and HIV. There was no cases positive for malaria parasite. Conclusion: The study reported a moderate prevalence of TTIs among blood donors, hence routine screening of all blood donors are crucial for the prevention and control of TTIs.