Introduction: Pain is a dehumanising sensation that diminishes the essence of a person. Pain is described as an unpleasant sensory and emotional experience linked to real or possible harm to body tissues or described in terms of such harm. There are various choices for managing pain after surgery, such as medications that affect the whole body (such as opioids and non-opioids), as well as treatments that target specific regions. Neostigmine is a commonly used drug to reverse the effects of neuromuscular block. Its ability to provide pain relief after surgery was initially reported by Naguib and Yaksh et al in 1994.Material and methods: The research was carried out in the anesthesia department of tertiary care hospitals affiliated with medical institutions in Haldia for a duration of one year. The study was carried out on 150 patients between the ages of 30 and 60 who were scheduled for hysterectomies. These patients had ASA grade I and II and gave their informed consent. Patients were divided into three groups, with each group consisting of 50 patients.Conclusion: The findings indicate that neostigimise prolongs the duration of pain relief provided by bupivicaine. Additionally, the use of transdermal nitroglycerine further enhances this postoperative pain relief. However, nitroglycerine alone does not possess any inherent analgesic properties. The use of intrathecal neostigmine and fentanyl in combination with bupivacaine resulted in a significant increase in postoperative pain relief compared to the control group, as well as the groups that received only bupivacaine and fentanyl or neostigmine.