Background: The present study was conducted for evaluating Mucosal changes occurring in gall stone patients.Materials &Methods: A total of 100 gallstone patients were enrolled. Complete demographic and clinical details of all the patients were obtained. A performa was made, and baseline hematological and biochemical findings were evaluated. Preoperative ultrasonographic evaluation of all the patients was done. All the patients underwent laparoscopic cholecystectomy was done. On examination, the size of gallstones was assessed. Gallbladder mucosal specimens were processed, and their H & E sections were evaluated. Histopathologic changes were evaluated. A correlation of histopathologic changes with gallstone thickness was done.Results: Mean age of the patients in the present study was 46.2 years. It was seen that cholecystitis was the final diagnosis in 79 percent of the patients while hyperplasia was the final diagnosis in 12 percent of the patients. Cholecystitis with metaplasia was the diagnosis in 6 percent of the patients whereas carcinoma was encountered in 3 percent of the patients. Mean gallbladder mucosal thickness among patients with cholecystitis, hyperplasia, metaplasia and carcinoma was 0.56 mm, 1.69 mm, 1.12 mm and 4.02 mm respectively. On comparing the results statistically, significant results were obtained. Conclusion: Diseases of the gallbladder can manifest in a variety of ways, both histologically and clinically. Therefore, in order to improve patient care, a thorough and rigorous macroscopic and microscopic evaluation of every cholecystectomy material should be required.