Background: Biofilms are ubiquitous in healthcare settings and are associated with health care associated infections. Biofilms are composed of complex microbial community embedded in an extracellular polymeric matrix (EPS). Bacteria commonly involved in biofilm formation are Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa. Formation of biofilm make the bacteria less susceptible for antimicrobial agents and disinfectants. Through cleaning practices with mechanical rubbing and use of appropriate disinfectant are needed to decrease the biofilm formation. Material & methods: 115 swabs were collected from ICU, Operation theater and wards. Swabs were cultured on Blood & MacConkey agar. Identification of organism were done as per standard microbiologic techniques. Antibiotic susceptibility done as per CLSI guidelines. Biofilm detection was done by Tube method described by Christensen et al.Result: 139 organisms were isolated of which Coagulase negative staphylococci were predominant (30.2% ) followed by Klebsiella spp,(22.3%). The predominant biofilm producing organism was Klebsiella spp (51.6%) followed by CONS.(33.3%). Both the organism were strong biofilm producer. Most of the oeganisms were multidrug resistant Conclusion: Hospital environment is colonised with biofilm producing bacteria. CONS & Klebsiella spp are common organism producing biofilms. Good cleaning practices are required to prevent biofilm formation.