Background: The study aimed to investigate the association between single nucleotide polymorphism (SNP) of the vitaminD receptor (VDR) gene(FokI,ApaI and TaqI) and benign prostatic hyperplasia (BPH) in North Indian men. Methods: The DNA wasextracted from the blood of 120 BPH patients and 120healthy age-matched controls and it was subjected to PCR followed by restriction fragment length polymorphism analysis. The odds ratio (OR) of having agenotype and developing BPH was calculated and the alleleswere designated risk-bearing or protective. Results:It was observed that Tt and Aa genotype was significantly low in BPH group as compared to healthy controls.No significant association was found with FokI polymorphism. Conclusion: This study suggests the protective role of heterozygous genotypes of TaqI and ApaI polymorphism against the development of BPH.