Background: Current antiviral regimens can cure up to 95% of persons with HCV infection and thus reduce the risk of death from cancer and liver cirrhosis, as well as the number of new infections. This means that screening and timely treatment of chronic hepatitis C (CHC) are the most promising measures in combating the epidemic of this infection. Eastern Europe and Central Asia (EECA) are among the regions with the highest prevalence of HCV infection. This study was conducted to assess the prevalence of hepatitis C virus in a known area. Material and methods: The study comprised a total of 500 participants. An automated chemiluminescent microparticle immunoassay was used to screen plasma samples for anti-HCV antibodies. The determination of Anti-HCV results relied on the signal-to-cutoff ratio (S/CO), with a threshold of S/CO ≥ 1.00 automatically classifying it as reactive using the Architect i1000SR platform. Prevlance of hepatitis C infection was recorded. All the results were subjected to statistical analysis using SPSS software.Results: Overall prevalence of Hepatitis C infection was 7.2 percent. Among these 36 patients, majority proportion were females (n=21). Most of the subjects with hepatitis C infection were of rural residence and belonged to the age group of more than 50 years. Conclusion: From the results of this study, it is evident that the prevalence of the disease was higher among the females as compared to males.