Background: Chronic otitis media is an inflammatory process in the middle ear space that result in long term or more often permanent changes in the tympanic membrane including- atelectasis, tympanosclerosis, retraction pocket formation or cholesteatoma. The primary symptom of chronic otitis media is conductive hearing loss but the patient can also present with otalgia, otorrhea, aural fullness, pulsatile tinnitus and otorrhagia. According to the American Academy of Ophthalmology and Otolaryngology Subcommittee on conservation of hearing (1965), Tympanoplasty is a procedure to eradicate disease in the middle ear and to reconstruct hearing mechanism with or without tympanic membrane grafting. Methods: This comparative study was carried out in the Department of Otorhinolaryngology, ASCOMS. About 50 patients (31 males; 19 females) were selected. These patients were divided randomly into 2 groups. Group-1 (25 patients) underwent tympanoplasty using temporals fascia graft and Group-2 (25 patients) underwent tympanoplasty using tragal perichondrium. Post operatively patients were assessed for complications at 1st month, 2nd month and 3rd month. Audiological (A-B Gap on PTA) and otoscopic examination was done to compare hearing improvement and healing of perforation. Results: Graft was taken up better in Group-1 (92.5%) as compared to Group-2 (87.5%). Hearing improvement was assessed by air-bone gap on PTA which was better in Group-2 as compared to Group-1 but it was not significant statistically. Conclusion: From our study, we suggest temporalis fascia has better graft uptake while the hearing improvement is equal in both the groups.