Objectives: Oxidative stress is the primary cause of sperm DNA damage and infertility, leading to potential risks such as pre- and post-implantation losses, congenital defects, and childhood cancer. Understanding oxidative stress origins and maintaining free radical levels is crucial for preventing these issues and improving male reproductive health. Materials and methods: The study conducted at Muzaffarnagar Medical College and Hospital focused on infertility treatment. It included a hospital-based observational study, outpatient department (OPD), inpatient department (IPD), and IVF center of the department of obstetrics and gynecology. The study lasted for 1 year and had a sample size of 50, based on the average size achieved in the previous five years. The participants were individuals attending the OPD for infertility treatment at Muzaffarnagar Medical College. The inclusion criteria were males with abnormal semen analysis without any other infertility factor in couples seeking infertility treatment, while female infertility patients were excluded from the study. Semen analysis and DNA fragmentation was performed to compare the results before and after the therapy. Results: Following the completion of the 90-day therapy period, a notable enhancement in the percentage of actively motile sperms was detected. On day 90, the active motility percentage was measured at 42.7%, accompanied by a standard deviation of 13.32. Throughout this duration, the observed values ranged from a minimum of 13% to a maximum of 61%. The calculated p-value for the active motility percentage was determined to be less than .00001 (p<0.05 significant). There was even significant decrease in DNA fragmentation index after 90 days therapy from 27.5% to 11.5%. Conclusion: Tablet zoafrag (protodioscin, Astaxanthin, Lycopene, L-carnitine, Coenzyme Q10 and selenomethionine) boosts ICSI, IVF, and IUI procedures by stimulating Sertoli and Leydig cells, increasing sperm production and converting testosterone to DHT. It improves acrosomal activity, allowing sperm to fuse with the oocyte membrane. It protects sperm PUFA, enhances sperm motility, and improves infertility. It also enhances sperm concentration and motility whereas decrease DNA fragmentation index after 90 days of therapy.