Background: The present study was conducted for assessing Iron, ferritin, TIBC and LDH levels in hypothyroid patients in Rohilkhand Region. Materials & Methods: A total of 100 hypothyroid patients were enrolled. Complete demographic and clinical details of all the patients were obtained. A Performa was made and complete medical and family history of all the patients was recorded separately. Another set of 100 age and gender matched healthy controls were also enrolled. Blood samples were obtained and Thyroid Profile, Iron, TIBC, LDH and Ferritin levels were evaluated among both healthy controls and hypothyroid patients. Results: Mean iron levels among patients of hypothyroid group and control group was 97.23 mcg/dL and 55.3 mcg/dL respectively. Mean ferritin levels among patients of hypothyroid group and control group were 105.1 ng/ml and 29.7 ng/ml respectively. Mean TIBC levels among patients of hypothyroid group and control group was 301.9 mcg/dL and 454.2 mcg/dL respectively. Mean LDH among patients of hypothyroid group and control group was 297.3 U/L and 498.3 U/L respectively. While comparing the results statistically between hypothyroid group and control group, significant results were obtained. Conclusion: Thyroid is affected with alteration in iron profile status along with LDH levels.