Background: Our modern lifestyle nowadays creating many hormonal disturbances one which is Diabetes Mellitus (DM) which is causing predisposition to many other diseases. Hence, it is necessary to detect these changes in their early stages. Aim: To establish correlation between periodontitis and diabetes mellitus patients by measuring alkaline phosphatise (ALP) in saliva. Material and Methodology: The saliva sample collected from patients and normal individual were analyzed biochemically using Spin react alkaline phosphatase (ALP) kit. Result: The ALP level increased in patients with diabetes mellitus and chronic periodontitis and was peaked in Diabetes mellitus type 2 with chronic periodontitis. Conclusion: As the ALP levels increased, it can be concluded that ALP has marked activity in diabetes mellitus and chronic periodontitis patients as these disease caused bone heath to be compromised.