Dramatic changes occur in the blood and bone marrow of the newborn during the first hours and days after birth and there are rapid fluctuations in the quantities of all hematologic elements. The values of most of the hematological parameters i.e mainly hemoglobin concentration, packed cell volume, reticulocyte count and red cell indices are highest on the first day of life and thereafter declines over the third day and the sixth week of life. There was daily visit to the Baby care clinic and postnatal care (PNC) ward. Neonates were examine in postnatal care ward & Baby care clinic and the parents were explain about the purpose of study. Those parents who satisfy inclusion criteria and ready for the study after counseling, their neonates were enrolled in the study and after taking informed consent from parents. Around 2 ml of venous blood was collected from baby under aseptic conditions and the sample was send to the lab for analysis. In the present study, the mean value of hemoglobin (Hb) was 13.1 ± 1.7 gm/dl (range 7.2 to 16.9 gm/dl), while 75.9% neonates had Hb level between 12-17 gm/dl and 24.1% had Hb level below 12 gm/dl. However, the mean values of Hb were significantly higher in 14-21 days old neonates compared to 22-28 days old neonates (p<0.05), but there was no significant difference between mean Hb levels of males and females.